Quantifying tumour-infiltrating lymphocyte subsets: A practical immuno-histochemical method
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چکیده
منابع مشابه
Quantification of tumour-infiltrating lymphocyte subsets: a practical immuno- histochemical method
Background A need for efficient quantification of tumour-infiltrating T and B lymphocytes (TIL) arose in designing a case-control study of breast cancer outcomes. Methods Ten adjacent x 40 digital images of 4μ sections immunostained for CD3, CD4, CD8, and CD20 were acquired for each of 16 randomly chosen carcinomas (640 images total). 'Color range' and 'Histogram' tools in Adobe Photoshop 7 wer...
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AIMS We aimed to analyze the phenotype of tumor-infiltrating lymphocytes (TILs) and non-tumor infiltrating lymphocytes (NILs) in HCC and non-tumor tissues, and evaluate relationships between changes in these cells and the prognosis of HCC. METHODS Lymphocytes were isolated from HCC and corresponding non-tumor tissues and tested by flow cytometry. For comparison, clinical parameters were analy...
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The rabbit has been widely used for the study of human atherosclerosis; however, the method for analysis of the atherosclerotic lesions has not been standardized between laboratories. The present study reports a practical method for quantifying the changes that occur in aortic atherosclerosis of rabbits. Male Japanese white rabbits were fed with either a standard chow or a diet containing 10% f...
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Background: Cytotoxic CD8+ T cells, as essential parts of the adaptive immune system, play pivotal roles in anti-tumor immune responses. It is well documented that cytokine expression profiles and activation status of these cells during anti-tumor immune responses affect the outcome of host-tumor interaction. Objective: To investigate the percentages of CD8+ lymphocytes and their subsets in...
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ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 2007
ISSN: 0022-1759
DOI: 10.1016/j.jim.2007.01.012